Journal: Frontiers in immunology
Article Title: Redox Regulator GLRX Is Associated With Tumor Immunity in Glioma.
doi: 10.3389/fimmu.2020.580934
Figure Lengend Snippet: FIGURE 7 | GLRX is specifically expressed by M0 macrophages. (A) The IHC co-localization staining of CD11b and GLRX. The red point represents GLRX, and the yellow area represents CD11b. Scale bar is 50 um (B) The proportion of stained cells. None represents cells without expression of CD11b and GLRX. Staining represents cells expressing CD11b and/or GLRX. CD11b represents cells expressing CD11b. GLRX represents cells expressing GLRX. CD11b + GLRX represents cells expressing CD11b and GLRX. (C) The left three photos are IF staining of M0 macrophages. Green fluorescence is GLRX, and red fluorescence is CD11b. Above is the merged photo of the below two. The right three photos are IF staining of M2 macrophages. Green fluorescence is GLRX, and red fluorescence is CD163. Above is the merged photo of the below two. Cell nuclei are stained with DAPI. Scale bar is 20 um. (D) The violin graph shows the proportion of GLRX- positive cells in M0 or M2 macrophage IF images. ****indicates p < 0.0001.
Article Snippet: IHC analysis with GLRX1 (Abcam, ab45953,1:1000) and CD11b antibodies (Proteintech, 66519-1-lg, 1:1000) was conducted according to standard procedures.
Techniques: Staining, Expressing